Recombinant Human RBM8A, His-tagged

• Cat.No.: RBM8A-30135TH
• Product Overview: Recombinant full length Human Y14 with a C terminal His tag; 182 amino acids with the tag, predicted mwt: 20.9 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 174 amino acids
• Description: This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.
• Conjugation: HIS
• Molecular Weight: 20.900kDa inclusive of tags
• Tissue specificity: Ubiquitous.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD GDEPGPQRSV EGWILFVTGV HEEATEEDIH DKFAEYGEIK NIHLNLDRRT GYLKGYTLVE YETYKEAQAA MEGLNGQDLM GQPISVDWCF VRGPPKGKRR GGRRRSRSPD RRRRLEHHHH HH
• Sequence Similarities: Belongs to the RBM8A family.Contains 1 RRM (RNA recognition motif) domain.

Gene Information

• Gene Name: RBM8A RNA binding motif protein 8A [ Homo sapiens ]
• Official Symbol: RBM8A
• Synonyms: RBM8A; RNA binding motif protein 8A; RBM8; RNA-binding protein 8A; BOV 1A; BOV 1B; BOV 1C; RBM8B; Y14; ZNRP
• Gene ID: 9939
• mRNA Refseq: NM_005105
• Protein Refseq: NP_005096
• MIM: 605313
• Uniprot ID: Q9Y5S9
• Chromosome Location: 1q21.1
• Pathway: Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem; Exon junction complex (EJC), organism-specific biosystem; Formation and Maturation of mRNA Transcript, organism-specific biosystem; Gene Expression, organism-specific biosystem; Metabolism, organism-specific biosystem
• Function: RNA binding; mRNA binding; nucleotide binding; protein binding