Recombinant Human RAD51

• Cat.No.: RAD51-134H
• Product Overview: The Active Full Length Human Rad51 protein was extensively purified from E. coli, which overexpressed the human Rad51 protein as a recombinant protein. Following the removal of the tag from the recombinant protein (though it retains Gly-Ser-His at the N-terminal), it has demonstrated maintained nuclear filament-forming and strand-exchange activities, along with an interaction capability with Rad52. This product has been verified to exhibit ATPase activity stimulated by single-strand DNA.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 1-339 a.a.
• Description: Human Rad51 protein is a functional and structural homolog of E. coli RecA protein, which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands. Rad51 functionally and physically interacts with its paralogs Dmc1, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3, and also with Rad52 in recombination processes. It also interacts with oncogene proteins and tumor suppressors such as BRCA1, BRCA2, and P53 for the maintenance of genome stability.
• Form: 20 mM Tris-HCl pH8.0, 100 mM KCl, 1 mM DTT, 0.5 mM EDTA, 10% glycerol
• Purity: >95% as judged from SDS-PAGE analysis
• Applications: 1) Studies on homologous recombination in mammals including human 2) Studies on the interaction of Rad51 protein with various proteins 3) To be used as a standard for Western blotting
• Storage: Sent at 4℃ or with dry-ice. Store at -80℃ for long period.
• Publications:
  Synthetic lethality triggered by combining Olaparib with BRCA2-Rad51 disruptors (2017)
  RAD51 Enhances Zygotic Interhomolog Repair (2018)
  Rad51/BRCA2 disruptors inhibit homologous recombination and synergize with olaparib in pancreatic cancer cells (2019)
  Efficient embryonic homozygous gene conversion via RAD51-enhanced interhomolog repair (2021)
  An 19F NMR fragment-based approach for the discovery and development of BRCA2-RAD51 inhibitors to pursuit synthetic lethality in combination with PARP inhibition in pancreatic cancer (2024)
  Investigating synthetic lethality and PARP inhibitor resistance in pancreatic cancer through enantiomer differential activity (2025)

Gene Information

• Gene Name: RAD51 RAD51 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: RAD51
• Synonyms: RAD51; RAD51 homolog (S. cerevisiae); RAD51 (S. cerevisiae) homolog (E coli RecA homolog) , RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) , RAD51A, RECA; DNA repair protein RAD51 homolog 1; BRCA1/BRCA2 containing complex; subunit 5; BRCC5; HsRad51; HsT16930; RAD51 homolog A; RecA-like protein; recombination protein A; RecA, E. coli, homolog of; RAD51 homolog (RecA homolog, E. coli); BRCA1/BRCA2-containing complex, subunit 5; RECA; MRMV2; HRAD51; RAD51A
• Gene ID: 5888
• mRNA Refseq: NM_001164269
• Protein Refseq: NP_001157741
• MIM: 179617
• UniProt ID: Q06609
• Chromosome Location: 15q15.1
• Pathway: Assembly of the RAD51-ssDNA nucleoprotein complex, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Homologous DNA pairing and strand exchange, organism-specific biosystem
• Function: ATP binding; ATP binding; DNA-dependent ATPase activity; damaged DNA binding; double-stranded DNA binding; double-stranded DNA binding; identical protein binding; nucleoside-triphosphatase activity; nucleotide binding; protein C-terminus binding; protein binding; single-stranded DNA binding; single-stranded DNA-dependent ATPase activity

Reviews

06/08/2024

RAD51-134H

Rad51 works well.