Recombinant Human QKI, KH domain containing, RNA binding, His-tagged

• Cat.No.: QKI-294H
• Product Overview: QKI, 1-341aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-341 a.a.
• Description: Protein quaking isoform HQK-5, also known as QKI, is an RNA-binding protein that regulates pre-mRNA splicing, export of mRNAs from the nucleus, protein translation, and mRNA stability. It is involved in myelinization and oligodendrocyte differentiation, and it may play a role in schizophrenia. Defects or deletions in the QKI are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Recombinant human QKI protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
• Molecular Mass: 40.1 kDa (364aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVGEMET KEKPKPTPDY LMQLMNDKKL MSSLPNFCGI FNHLERLLDE EISRVRKDMY NDTLNGSTEK RSAELPDAVG PIVQLQEKLY VPVKEYPDFN FVGRILGPRG LTAKQLEAET GCKIMVRGKG SMRDKKKEEQ NRGKPNWEHL NEDLHVLITV EDAQNRAEIK LKRAVEEVKK LLVPAAEGED SLKKMQLMEL AILNGTYRDA NIKSPALAFS LAATAQAAPR IITGPAPVLP PAALRTPTPA GPTIMPLIRQ IQTAVMPNGT PHPTAAIVPP GPEAGLIYTP YEYPYTLAPA TSILEYPIEP SGVLGAVATK VRRHDMRVHP YQRIVTADRA ATGN
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: QKI QKI, KH domain containing, RNA binding [ Homo sapiens ]
• Official Symbol: QKI
• Synonyms: QKI; QKI, KH domain containing, RNA binding; quaking homolog, KH domain RNA binding (mouse); protein quaking; QK3; RNA binding protein HQK; quaking homolog, KH domain RNA binding; homolog of mouse quaking QKI (KH domain RNA binding protein); QK; Hqk; QK1; hqkI; DKFZp586I0923
• Gene ID: 9444
• mRNA Refseq: NM_006775
• Protein Refseq: NP_006766
• MIM: 609590
• UniProt ID: Q96PU8
• Chromosome Location: 6q26
• Pathway: Diurnally regulated genes with circadian orthologs, organism-specific biosystem
• Function: RNA binding; SH3 domain binding; protein binding