Active Recombinant Human PVRL2 Protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: PVRL2-611H
• Product Overview: Biotinylated Human Nectin-2, Fc Tag is expressed from HEK293 cells. It contains AA Gln 32 - Leu 360 (Accession # Q92692-2). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 32-360 a.a.
• Predicted N Terminal: Gln 32
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human TIGIT, Fc Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Human Nectin-2, Fc Tag with a linear range of 0.313-10 ug/mL. Immobilized Human DNAM-1, Fc Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Human Nectin-2, Fc Tag with a linear range of 0.156-5 ug/mL.
• Molecular Mass: The protein has a calculated MW of 64.0 kDa. As a result of glycosylation, the protein migrates as 66 kDa under reducing condition, and 130 kDa under non-reducing condition (SDS-PAGE).
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PVRL2 poliovirus receptor-related 2 (herpesvirus entry mediator B) [ Homo sapiens ]
• Official Symbol: PVRL2
• Synonyms: PVRL2; poliovirus receptor-related 2 (herpesvirus entry mediator B); HVEB; poliovirus receptor-related protein 2; CD112; PRR2; PVRR2; nectin-2; poliovirus receptor-like 2; herpesvirus entry protein B
• Gene ID: 5819
• mRNA Refseq: NM_001042724
• Protein Refseq: NP_001036189
• MIM: 600798
• UniProt ID: Q92692