Recombinant Human PVRIG protein(Thr41-Leu172), hFc&Avi-tagged, Biotinylated

• Cat.No.: PVRIG-2395H
• Product Overview: Recombinant Human PVRIG (Q6DKI7-1)(Thr41-Leu172) was expressed in HEK293 with a c-terminal AVI tagged Fc region of Human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: Thr41-Leu172
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human Nectin-2 hFc at 2 μg/mL (100 μL/well) can bind Human PVRIG (hFc & AVI Tag), Biotinylated, the EC50 of Human PVRIG (hFc & AVI Tag), Biotinylated is 40-220 ng/mL.
• Molecular Mass: The recombinant human PVRIG consists of 385 amino acids and predicts a molecular mass of 42.31 kDa. It migrates as an approximately 58.43 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PVRIG poliovirus receptor related immunoglobulin domain containing [ Homo sapiens ]
• Official Symbol: PVRIG
• Synonyms: PVRIG; poliovirus receptor related immunoglobulin domain containing; transmembrane protein PVRIG; C7orf15; MGC2463; poliovirus receptor-related immunoglobulin domain-containing protein; MGC104322; MGC138295; MGC138297
• Gene ID: 79037
• mRNA Refseq: NM_024070
• Protein Refseq: NP_076975
• UniProt ID: Q6DKI7