Recombinant Human PTN protein, His-tagged

• Cat.No.: PTN-439H
• Product Overview: Recombinant Human PTN (P21246) aa. (Gly33~Asp168) fused with His tag was produced in HEK293F cells.

Specification

• Description: The protein encoded by this gene is a secreted heparin-binding growth factor. The protein has significant roles in cell growth and survival, cell migration, angiogenesis and tumorigenesis. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
• Source: HEK293F
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.7kDa; Accurate Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gly33~Asp168
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Characteristic: Isoelectric point is 9.6.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: PTN pleiotrophin [ Homo sapiens (human) ]
• Official Symbol: PTN
• Synonyms: PTN; pleiotrophin; HARP; HBBM; HBNF; HBGF8; NEGF1; OSF-1; HB-GAM; HBGF-8; HBNF-1; heparin affin regulatory protein; heparin-binding brain mitogen; heparin-binding growth factor 8; heparin-binding growth-associated molecule; heparin-binding neurite outgrowth promoting factor; heparin-binding neurite outgrowth-promoting factor 1; osteoblast-specific factor 1; pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)
• Gene ID: 5764
• mRNA Refseq: NM_001321386.2
• Protein Refseq: NP_001308315.1
• UniProt ID: P21246