Recombinant Human PTH1R protein, His-tagged

• Cat.No.: PTH1R-8043H
• Product Overview: Recombinant Human PTH1R aa. (Ala28~Gly188) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ala28~Gly188
• Description: The protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis. Two transcript variants encoding the same protein have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 6.6.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PTH1R parathyroid hormone 1 receptor [ Homo sapiens (human) ]
• Official Symbol: PTH1R
• Synonyms: PFE; PTHR; PTHR1; PTH/PTHr receptor; PTH/PTHrP type I receptor; PTH1 receptor; parathyroid hormone receptor 1; parathyroid hormone/parathyroid hormone-related protein receptor; seven transmembrane helix receptor; parathyroid hormone/parathyroid hormone-related peptide receptor
• Gene ID: 5745
• mRNA Refseq: NM_000316.2
• Protein Refseq: NP_000307.1
• UniProt ID: Q03431