Recombinant Human PSMC3 Protein, His-tagged

• Cat.No.: PSMC3-420H
• Product Overview: Recombinant Human PSMC3(Asn2~Ala439) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Asn2~Ala439
• Description: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 52.7kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 98%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: PSMC3 proteasome (prosome, macropain) 26S subunit, ATPase, 3 [ Homo sapiens ]
• Official Symbol: PSMC3
• Synonyms: PSMC3; proteasome (prosome, macropain) 26S subunit, ATPase, 3; 26S protease regulatory subunit 6A; TBP 1; TBP1; TBP-1; Tat-binding protein 1; proteasome subunit P50; proteasome 26S subunit ATPase 3; 26S proteasome AAA-ATPase subunit RPT5; human immunodeficiency virus tat transactivator binding protein-1; MGC8487
• Gene ID: 5702
• mRNA Refseq: NM_002804
• Protein Refseq: NP_002795
• MIM: 186852
• UniProt ID: P17980