Recombinant Human PSMB4 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PSMB4-1545H
• Product Overview: PSMB4 MS Standard C13 and N15-labeled recombinant protein (NP_002787) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 29 kDa
• AA Sequence: MEAFLGSRSGLWAGGPAPGQFYRIPSTPDSFMDPASALYRGPITRTQNPMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARDLVERCMRVLYYRDARSYNRFQIATVTEKGVEIEGPLSTETNWDIAHMISGFETRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PSMB4 proteasome 20S subunit beta 4 [ Homo sapiens (human) ]
• Official Symbol: PSMB4
• Synonyms: PSMB4; proteasome (prosome, macropain) subunit, beta type, 4; proteasome subunit beta type-4; HN3; PROS26; hsBPROS26; proteasome chain 3; macropain beta chain; proteasome beta chain; 26 kDa prosomal protein; proteasome subunit HsN3; proteasome subunit, beta type, 4; multicatalytic endopeptidase complex beta chain; HsN3; PROS-26
• Gene ID: 5692
• mRNA Refseq: NM_002796
• Protein Refseq: NP_002787
• MIM: 602177
• UniProt ID: P28070