Recombinant Human PRSS3 Protein, His-tagged, Active

• Cat.No.: PRSS3-517H
• Product Overview: Recombinant Human PRSS3(Ile24-Ser247) fused with His tag at C-terminal was expressed in NS0.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Ile24-Ser247
• Description: Human Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin. Constituting less than 10% of the total trypsin content in normal pancreatic juice, it is one of the three trypsin isoforms produced by the pancreas. Compared to trypsin 1 and 2, one intriguing feature of Trypsin 3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. As revealed by the crystal structure, this resistance is likely due to the presence of an arginine residue in place of the highly conserved Gly198. Trypsin 3 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. One proposed physiological function of Trypsin 3 is degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins.
• Predicted N Terminal: Ile24
• Form: Supplied as a 0.2 μm filtered solution in HCl, NaCl, CaCl2 and Brij-35.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >4,000 pmol/min/µg.
• Molecular Mass: Predicted Molecular Mass: 26 kDa;
  SDS-PAGE: 20-24 kDa, non-reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: PRSS3 protease, serine, 3 [ Homo sapiens ]
• Official Symbol: PRSS3
• Synonyms: PRSS3; protease, serine, 3; protease, serine, 3 (mesotrypsin) , protease, serine, 4 (trypsin 4, brain) , PRSS4; trypsin-3; mesotrypsin; TRY3; TRY4; trypsin IV; trypsin III; trypsinogen 4; trypsinogen 5; trypsinogen IV; mesotrypsinogen; brain trypsinogen; pancreatic trypsinogen III; protease, serine, 4 (trypsin 4, brain); T9; MTG; PRSS4
• Gene ID: 5646
• mRNA Refseq: NM_001197097
• Protein Refseq: NP_001184026
• MIM: 613578
• UniProt ID: P35030