Recombinant Human Protein Tyrosine Phosphatase, Non-Receptor Type 9

• Cat.No.: PTPN9-1910H
• Product Overview: Recombinant humanPTPN9 is expressed in E. coli; it contains the catalytic domain of PTPN9(residues 285-593) and has a molecular weight of 34.3 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 285-593 a.a.
• Description: PTPN9 (proteintyrosine phosphatase from megakaryocyte-2) was originally cloned from humanMEG-01 megakaryocyte and umbilical vein endothelial cell cDNA. The catalyticdomain is located at the carboxy-terminus of the full-length protein and ithas 30-40% sequence identity to other known protein tyrosine phosphatases.The full-length enzyme has been reported to bind phospholipid with highaffinity and that this binding is important for MEG2 activity on secretoryvesicles.
• Form: 25 mM Tris-HCl (pH8.0), 75 mM NaCl, 0.05% Tween-20, 2 mM EDTA, 1 mM DTT and 50% glycerol.Liquid at -20°C.
• Purity: Greater than 95% asdetermined by Coomassie-stained SDS-PAGE gel.
• Specific Activity: 3.5 U/μg. One unitwill hydrolyze 1 nmol of p-nitrophenyl phosphate per minute at pH 7.4 and30°C. Assay buffer: 50 mM HEPES (pH 7.4), 2 mM EDTA, 3 mM DTT, 100 mM NaCland 50 mM pNPP.
• Applications: Removal ofphosphates from target substrates. Useful for the study of enzyme kinetics,regulation and for inhibitors screening.
• Storage: 6 months at -20°C.

Gene Information

• Gene Name: PTPN9 protein tyrosinephosphatase, non-receptor type 9 [ Homo sapiens ]
• Official Symbol: PTPN9
• Synonyms: PTPN9; proteintyrosine phosphatase, non-receptor type 9; MEG2; PTPMEG2; tyrosine-proteinphosphatase non-receptor type 9; PTPase MEG2; PTPase-MEG2; OTTHUMP00000183522;protein-tyrosine phosphatase MEG2
• Gene ID: 5780
• mRNA Refseq: NM_002833
• Protein Refseq: NP_002824
• MIM: 600768
• UniProt ID: P43378
• Chromosome Location: 15q23
• Function: hydrolase activity;non-membrane spanning protein tyrosine phosphatase activity; protein binding;protein tyrosine phosphatase activity