Recombinant Human Proteasome (Prosome, Macropain) Subunit, Beta Type, 3, His-tagged

• Cat.No.: PSMB3-1540H
• Product Overview: Recombinant human PSMB3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: PSMB3-1540H
• Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl
• Molecular Weight: 25.1 kDa (225aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSIMSYNGGA VMAMKGKNCV AIAADRRFGI QAQMVTTDFQ KIFPMGDRLY IGLAGLATDV QTVAQRLKFR LNLYELKEGR QIKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP DHLFETISQA MLNAVDRDAV SGMGVIVHII EKDKITTRTL KARMD
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: APC/C-mediated degradation of cell cycle proteins; APC/C:Cdc20 mediated degradation of Securin; Adaptive Immunity Signaling; Apoptosis; Assembly of the pre-replicative complex; Autodegradation of the E3 ubiquitin ligase COP1; CDK-mediated phosphorylation and removal of Cdc6; CDT1 association with the CDC6:ORC:origin complex; Cell Cycle Checkpoints; Cell Cycle, Mitotic; Cyclin A:Cdk2-associated events at S phase entry; Cyclin E associated events during G1/S transition; Vpu mediated degradation of CD4; Vif-mediated degradation of APOBEC3G; Synthesis of DNA

Gene Information

• Gene Name: PSMB3 proteasome (prosome, macropain) subunit, beta type, 3 [ Homo sapiens ]
• Official Symbol: PSMB3
• Synonyms: PSMB3; HC10-II; MGC4147; proteasome subunit beta type-3; OTTHUMP00000164161; proteasome chain 13; proteasome theta chain; proteasome component C10-II; proteasome (prosome, macropain) subunit, beta type, 3; Proteasome chain 13; Proteasome component C10-II; Proteasome theta chain; EC 3.4.25.1
• Gene ID: 5691
• mRNA Refseq: NM_002795
• Protein Refseq: NP_002786
• MIM: 602176
• UniProt ID: P49720
• Chromosome Location: 17q12
• Function: peptidase activity; protein binding; threonine-type endopeptidase activity