Recombinant Full Length Human PRKCQ Protein, GST tagged

• Cat.No.: PRKCQ-30178TH
• Product Overview: Recombinant full-length human PKC theta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-706 aa
• Description: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.
• Source: Sf9 insect cell
• Species: Human
• Tag: N-GST
• Purity: >90%
• Stability: 1 year at –70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Storage Buffer: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Concentration: 0.1 μg/μL
• Shipping: Dry ice
• Reference: 1. Manicassamy, S. and Sun, Z. The critical role of protein kinase C-theta in Fas/Fas ligand-mediated apoptosis. J. Immunol. 2007;178(1):312-9.
  2. Kim, J K. et al: PKC-theta knockout mice are protected from fat-induced insulin resistance. J. Clin. Invest. 2004;114(6):823-7

Gene Information

• Gene Name: PRKCQ protein kinase C, theta [ Homo sapiens ]
• Official Symbol: PRKCQ
• Synonyms: PRKCQ; protein kinase C, theta; protein kinase C theta type; PRKCT; nPKC-theta; MGC126514; MGC141919
• Gene ID: 5588
• mRNA Refseq: NM_006257
• Protein Refseq: NP_006248
• MIM: 600448
• UniProt ID: Q04759