Recombinant Human PRKCI, His&T7-tagged

• Cat.No.: PRKCI-406H
• Product Overview: Recombinant Human PRKCI(Ile375~Val596), fused with two N-terminal Tags, His-tag and T7-tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Ile375~Val596
• Description: This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Molecular Mass: 29.1kDa
• AA Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IYRDLK LDNVLLDSEG HIKLTDYGMC KEGLRPGDTT STFCGTPNYI APEILRGEDY GFSVDWWALG VLMFEMMAGR SPFDIVGSSD NPDQNTEDYL FQVILEKQIR IPRSLSVKAA SVLKSFLNKD PKERLGCHPQ TGFADIQGHP FFRNVDWDMM EQKQVVPPFK PNISGEFGLD NFDSQFTNEP VQLTPDDDDI VRKIDQSEFE GFEYINPLLM SAEECV
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37ºC for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months.

Gene Information

• Gene Name: PRKCI protein kinase C, iota [ Homo sapiens (human) ]
• Official Symbol: PRKCI
• Synonyms: PRKCI; protein kinase C, iota; PKCI; DXS1179E; MGC26534; nPKC-iota; EC 2.7.11.13; Protein kinase C iota type; Atypical protein kinase C-lambda/iota; aPKC-lambda/iota; PRKC-lambda/iota
• Gene ID: 5584
• mRNA Refseq: NM_002740
• Protein Refseq: NP_002731
• MIM: 600539
• UniProt ID: P41743
• Chromosome Location: 3q26.3
• Pathway: Cell junction organization; Corticotropin-releasing hormone; G Protein Signaling Pathways
• Function: ATP binding; metal ion binding; nucleotide binding; protein domain specific binding