Active Recombinant Human PRKAG1 & PRKAB2 & PRKAA1 Protein, His-GST-tagged

• Cat.No.: PRKAG1 & PRKAB2 & PRKAA1-1238H
• Product Overview: Recombinant human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; Recombinant human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; Recombinant human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tag

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-331;1-272 a.a.
• Predicted N Terminal: Met & Met & His
• Form: Supplied as sterile 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% gly, pH 7.4.
• Bio-activity: The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
• Molecular Mass: The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >94 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: PRKAG1 protein kinase, AMP-activated, gamma 1 non-catalytic subunit [ Homo sapiens ]
• Official Symbol: PRKAG1
• Synonyms: PRKAG1; protein kinase, AMP-activated, gamma 1 non-catalytic subunit; 5-AMP-activated protein kinase subunit gamma-1; AMPK gamma1; AMPK gamma-1 chain; 5-AMP-activated protein kinase, gamma-1 subunit; AMPKG; MGC8666
• Gene ID: 5571
• mRNA Refseq: NM_001206709
• Protein Refseq: NP_001193638
• MIM: 602742
• UniProt ID: P54619
• Chromosome Location: 12q12-q14
• Pathway: AMPK signaling, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Energy Metabolism, organism-specific biosystem; Energy dependent regulation of mTOR by LKB1-AM
• Function: ADP binding; AMP binding; contributes_to AMP-activated protein kinase activity; AMP-activated protein kinase activity; ATP binding; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase regulator activity; nucleotide binding; protein bindi