Recombinant Human PRIM2 protein, His-tagged

• Cat.No.: PRIM2-3242H
• Product Overview: Recombinant Human PRIM2 protein(1-158 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 1-158 aa
• AA Sequence: MEFSGRKWRKLRLAGDQRNASYPHCLQFYLQPPSENISLIEFENLAIDRVKLLKSVENLGVSYVKGTEQYQSKLESELRKLKFSYRENLEDEYEPRRRDHISHFILRLAYCQSEELRRWFIQQEMDLLRFRFSILPKDKIQDFLKDSQLQFEAVSIFL
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: PRIM2 primase, DNA, polypeptide 2 (58kDa) [ Homo sapiens ]
• Official Symbol: PRIM2
• Synonyms: PRIM2; primase, DNA, polypeptide 2 (58kDa); PRIM2A, primase, polypeptide 2A (58kD) , primase, polypeptide 2A, 58kDa; DNA primase large subunit; dJ422B11.1.1; DNA primase subunit p58; DNA primase 58 kDa subunit; primase polypeptide 2A, 58kDa; p58; PRIM2A; MGC75142
• Gene ID: 5558
• mRNA Refseq: NM_000947
• Protein Refseq: NP_000938
• MIM: 176636
• UniProt ID: P49643