Recombinant Human PRDM9 Protein, His-tagged

• Cat.No.: PRDM9-024H
• Product Overview: Recombinant Human PRDM9 protein (2-511) with a N-terminal His-tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-511
• Description: The protein encoded by this gene is a zinc finger protein with histone methyltransferase activity that catalyzes histone H3 lysine 4 trimethylation (H3K4me3) during meiotic prophase. This protein contains multiple domains, including a Kruppel-associated box (KRAB) domain, an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain, and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. The observed allelic variation alters the DNA-binding sequence specificity of the protein, resulting in distinct meiotic recombination hotspots amongst individuals and populations. Multiple alternate alleles of this gene have been described.
• Molecular Mass: 60.5 kDa
• Purity: ≥30% estimated by SDS-PAGE
• Stability: ≥ 9 months
• Storage: -80 centigrade
• Storage Buffer: 50 mM Tris-HCl, pH 8.0, containing 150 mM sodium chloride and 20% glycerol

Gene Information

• Gene Name: PRDM9 PR/SET domain 9 [ Homo sapiens (human) ]
• Official Symbol: PRDM9
• Synonyms: PRDM9; PR/SET domain 9; PFM6; KMT8B; MSBP3; ZNF899; MEISETZ; histone-lysine N-methyltransferase PRDM9; PR domain 9; PR domain containing 9; PR domain zinc finger protein 9; [histone H3]-lysine36 N-trimethyltransferase PRDM9; [histone H3]-lysine4 N-trimethyltransferase PRDM9; [histone H3]-lysine9 N-trimethyltransferase PRDM9; [histone H4]-N-methyl-L-lysine20 N-methyltransferase PRDM9; [histone H4]-lysine20 N-methyltransferase PRDM9; histone methyl transferase; minisatellite binding protein 3 (115kD); protein-lysine N-methyltransferase PRDM9; EC 2.1.1.354
• Gene ID: 56979
• mRNA Refseq: NM_001310214
• Protein Refseq: NP_001297143
• MIM: 609760
• UniProt ID: Q9NQV7