Recombinant Human polo-like kinase 4 Protein, His tagged

• Cat.No.: PLK4-203H
• Product Overview: Recombinant human PLK4 protein (O00444-1) (Glu 581-Ser 812) with a His tag at the C-terminus was expressed in E. coli.

Specification

• Description: Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification.
• Source: E. coli
• Species: Human
• Tag: C-His
• Protein length: 581-812aa
• Molecular Mass: 28.4 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: PLK4 polo-like kinase 4 [ Homo sapiens (human) ]
• Official Symbol: PLK4
• Synonyms: PLK4; polo-like kinase 4; polo like kinase 4 (Drosophila), serine/threonine kinase 18, STK18; serine/threonine-protein kinase PLK4; Sak; PLK-4; Snk akin kinase; serine/threonine kinase 18; serine/threonine-protein kinase 18; serine/threonine protein kinase SAK; serine/threonine-protein kinase Sak; SAK; STK18
• Gene ID: 10733
• mRNA Refseq: NM_001190799
• Protein Refseq: NP_001177728
• MIM: 605031
• UniProt ID: O00444