Recombinant Human PMP22 protein, His & T7-tagged

• Cat.No.: PMP22-8045H
• Product Overview: Recombinant Human PMP22 aa. (Met1~Glu160 (Accession # Q01453)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Met1~Glu160
• Description: This gene encodes an integral membrane protein that is a major component of myelin in the peripheral nervous system. Studies suggest two alternately used promoters drive tissue-specific expression. Various mutations of this gene are causes of Charcot-Marie-Tooth disease Type IA, Dejerine-Sottas syndrome, and hereditary neuropathy with liability to pressure palsies. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.4.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PMP22 peripheral myelin protein 22 [ Homo sapiens (human) ]
• Official Symbol: PMP22
• Synonyms: DSS; CIDP; GAS3; HNPP; CMT1A; CMT1E; GAS-3; Sp110; HMSNIA; growth arrest-specific protein 3; peripheral myelin protein 22 kDa; peripheral myelin protein 22
• Gene ID: 5376
• mRNA Refseq: NM_000304.3
• Protein Refseq: NP_000295.1
• UniProt ID: Q01453