Recombinant Human PLCH1 protein, His & T7-tagged

• Cat.No.: PLCH1-1023H
• Product Overview: Recombinant Human PLCH1 aa. (Met1~Ala237 (Accession # Q4KWH8) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.6kDa.
• Protein length: Met1~Ala237
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 6.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: PLCH1 phospholipase C eta 1 [ Homo sapiens (human) ]
• Official Symbol: PLCH1
• Synonyms: PLCH1; phospholipase C eta 1; PLCL3; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-1; PLC eta 1; PLC-L3; phosphoinositide phospholipase C-eta-1; phospholipase C-eta1a; phospholipase C-eta1b; phospholipase C-like 3; phospholipase C-like protein 3
• Gene ID: 23007
• mRNA Refseq: NM_001130960.1
• Protein Refseq: NP_001124432.1
• UniProt ID: Q4KWH8