Recombinant Human PLCG1, MYC/DDK-tagged

• Cat.No.: PLCG1-17H
• Product Overview: Recombinant Human PLCG1, fused with C-terminal MYC/DDK, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
• Molecular Mass: 148.5 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: PLCG1 phospholipase C, gamma 1 [ Homo sapiens ]
• Official Symbol: PLCG1
• Synonyms: PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1; phospholipase C, gamma 1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; PLC-gamma-1; phospholipase C-II; phospholipase C-148; phosphoinositidase C; phospholipase C-gamma-1; inositoltrisphosphohydrolase; phosphoinositide phospholipase C; phosphatidylinositol phospholipase C; triphosphoinositide phosphodiesterase; phosphoinositide phospholipase C-gamma-1; monophosphatidylinositol phosphodiesterase; 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; phospholipase C, gamma 1 (formerly subtype 148); 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; NP_002651.2; EC 3.1.4.11
• Gene ID: 5335
• mRNA Refseq: NM_002660
• Protein Refseq: NP_002651
• MIM: 172420
• UniProt ID: P19174
• Chromosome Location: 20q12-q13.1
• Pathway: Adaptive Immune System; Antiviral mechanism by IFN-stimulated genes; Calcium signaling pathway
• Function: calcium ion binding; glutamate receptor binding; phosphatidylinositol phospholipase C activity