Recombinant Human PIP4K2B, His-tagged

• Cat.No.: PIP4K2B-7242H
• Product Overview: Recombinant human PIP4K2B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Specification

• Species: Human
• Source: E.coli
• Tag: His
• description: PIP4K2B catalyzes the phosphorylation of phosphatidylinositol-4-phosphate on the fifth hydroxyl of the myo-inositol ring to form phosphatidylinositol-4,5-bisphosphate. It is a member of the phosphatidylinositol-4-phosphate 5-kinase family. The amino acid sequence of PIP4K2B does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, PIP4K2B interacts with p55 TNF receptor.
• source: E. coli
• species: Human
• tag: His
• form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• molecular mass: 49.8 kDa (439aa)
• aa sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSNCTS TTAVAVAPLS ASKTKTKKKH FVCQKVKLFR ASEPILSVLM WGVNHTINEL SNVPVPVMLMPDDFKAYSKI KVDNHLFNKE NLPSRFKFKE YCPMVFRNLR ERFGIDDQDY QNSVTRSAPI NSDSQGRCGT RFLTTYDRRF VIKTVSSEDV AEMHNILKKY HQFIVECHGN TLLPQFLGMY RLTVDGVETY MVVTRNVFSH RLTVHRKYDL KGSTVAREAS DKEKAKDLPT FKDNDFLNEGQKLHVGEESK KNFLEKLKRD VEFLAQLKIM DYSLLVGIHD VDRAEQEEME VEERAEDEEC ENDGVGGNLL CSYGTPPDSP GNLLSFPRFF GPGEFDPSVD VYAMKSHESS PKKEVYFMAI IDILTPYDTK KKAAHAAKTV KHGAGAEIST VNPEQYSKRF NEFMSNILT
• purity: >90% by SDS - PAGE
• applications: SDS-PAGE
• storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• concentration: 0.5 mg/ml

Gene Information

• gene name: PIP4K2B phosphatidylinositol-5-phosphate 4-kinase, type II, beta [ Homo sapiens ]
• official symbol: PIP4K2B
• synonyms: PIP4K2B; phosphatidylinositol-5-phosphate 4-kinase, type II, beta; phosphatidylinositol 4 phosphate 5 kinase, type II, beta , PIP5K2B; phosphatidylinositol-5-phosphate 4-kinase type-2 beta; PIP5KIIB; PIP5KIIbeta; PIP4KII-beta; PTDINS(4)P-5-kinase; PI(5)P 4-kinase type II beta; diphosphoinositide kinase 2-beta; ptdIns(5)P-4-kinase isoform 2-beta; 1-phosphatidylinositol-4-phosphate kinase; 1-phosphatidylinositol-5-phosphate 4-kinase 2-beta; phosphatidylinositol-4-phosphate 5-kinase, type II, beta; PI5P4KB; PIP5K2B
• Gene ID: 8396
• mrna refseq: NM_003559
• protein refseq: NP_003550
• mim: 603261
• uniprot id: P78356
• chromosome location: 17q21.2
• pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, organism-specific biosystem; Inositol phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, conserved biosystem; Phosphatidylinositol signaling system, organism-specific biosystem; Phosphatidylinositol signaling system, conserved biosystem
• function: 1-phosphatidylinositol-4-phosphate 5-kinase activity; 1-phosphatidylinositol-5-phosphate 4-kinase activity; ATP binding; nucleotide binding; receptor signaling protein activity; transferase activity