Recombinant Human PIP4K2B

• Cat.No.: PIP4K2B-188H

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Description: The protein catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-4,5- bisphosphate. The gene is a member of the phosphatidylinositol-4- phosphate 5-kinase family. The encoded protein sequence does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, the encoded protein interacts with p55 TNF receptor. Two transcript variants encoding different isoforms have been found for this gene. Pip5k2 beta, is amplified in a subset of breast cancer cell lines and primary breast cancer samples that carry HER-2/Neu amplification. It has been implicated that Pip4k2beta has a role in the development and/or progression of breast cancer. The PIP5Kβ was expressed in a Baculovirus system and purified by affinity and FPLC chromatography. MW = 46 kDa.
• Form: Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA.
• Purity: > 95% by SDS-PAGE.
• Application: 1 μg of PIP5K2βis sufficient to phosphorylate 1 μg of heat-inactivated nuclear or cytoplasmic extract.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: PIP4K2B phosphatidylinositol-5-phosphate 4-kinase, type II, beta [ Homo sapiens ]
• Synonyms: PIP4K2B; phosphatidylinositol-5-phosphate 4-kinase, type II, beta; PI5P4KB; PIP5K2B; PIP5KIIB; PIP5KIIbeta; 1-phosphatidylinositol-5-phosphate 4-kinase B; PTDINS(4)P-5-kinase diphosphoinositide kinase; 1-phosphatidylinositol-4-phosphate kinase; phosphatidylinositol-4-phosphate 5-kinase, type II, beta; EC 2.7.1.149; Phosphatidylinositol-5-phosphate 4-kinase type-2 beta; PIP4KII-beta; Diphosphoinositide kinase 2-beta; PIP4KII-beta
• Gene ID: 8396
• mRNA Refseq: NM_003559
• Protein Refseq: NP_003550
• MIM: 603261
• UniProt ID: P78356
• Chromosome Location: 17q21.2
• Pathway: Endocytosis; Fc gamma R-mediated phagocytosis; Inositol phosphate metabolism; Metabolic pathways; Phosphatidylinositol signaling system; Regulation of actin cytoskeleton
• Function: 1-phosphatidylinositol-4-phosphate 5-kinase activity; 1-phosphatidylinositol-5-phosphate 4-kinase activity; ATP binding; kinase activity; nucleotide binding; protein binding; receptor signaling protein activity; transferase activity