Recombinant Human PINK1 protein, His-tagged

• Cat.No.: PINK1-3194H
• Product Overview: Recombinant Human PINK1 protein(150-300 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 150-300 aa
• AA Sequence: GFRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAAGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLP
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: PINK1 PTEN induced putative kinase 1 [ Homo sapiens ]
• Official Symbol: PINK1
• Synonyms: PINK1; PTEN induced putative kinase 1; PARK6, Parkinson disease (autosomal recessive) 6; serine/threonine-protein kinase PINK1, mitochondrial; protein kinase BRPK; PTEN-induced putative kinase protein 1; BRPK; PARK6; FLJ27236
• Gene ID: 65018
• mRNA Refseq: NM_032409
• Protein Refseq: NP_115785
• MIM: 608309
• UniProt ID: Q9BXM7