Recombinant Human Phosphoinositide-3-kinase, Catalytic, Alpha Polypeptide, His-tagged

• Cat.No.: PIK3CA-1078H
• Product Overview: Recombinant full-length human p110 alpha (E545K) mutant subunit and human p85 alpha wild-type subunit were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: The PIK3CA comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110α catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon and this protein is one of the PIK3CA mutants. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110α is essential for vascular development and inactivation of p110α leads to severe defects in angiogenic sprouting and vascular remodeling.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 111 kDa (p110α), 86 kDa (p85α)
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
• Specific Activity: 50 nmol/min/mg
• Purity: > 70 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: 3-phosphoinositide biosynthesis; Acute myeloid leukemia; Acute myeloid leukemia; Adaptive Immunity Signaling; B cell receptor signaling pathway; Bacterial invasion of epithelial cells; CDC42 signaling events; Downstream signaling of activated FGFR; EPHA2 forward signaling; FGF signaling pathway; HTLV-I infection; IL23-mediated signaling events; Inositol phosphate metabolism; Jak-STAT signaling pathway; Non-small cell lung cancer

Gene Information

• Gene Name: PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide [ Homo sapiens ]
• Official Symbol: PIK3CA
• Synonyms: PIK3CA; phosphoinositide-3-kinase, catalytic, alpha polypeptide; PI3K; MGC142161; MGC142163; p110-alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3K-alpha; OTTHUMP00000211945; PtdIns-3-kinase p110; PI3-kinase subunit alpha; PI3-kinase p110 subunit alpha; ptdIns-3-kinase subunit alpha; ptdIns-3-kinase subunit p110-alpha; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
• Gene ID: 5290
• mRNA Refseq: NM_006218
• Protein Refseq: NP_006209
• MIM: 171834
• UniProt ID: P42336
• Chromosome Location: 3q26.3
• Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; inositol or phosphatidylinositol kinase activity; insulin receptor substrate binding; nucleotide binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor; protein binding; protein serine/threonine kinase activity