Recombinant Human Phosphogluconate Dehydrogenase, His-tagged

• Cat.No.: PGD-2034H
• Product Overview: Recombinant human Phosphogluconate Dehydrogenase protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW=55.3 kDa (503aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PGD (Phosphogluconate dehydrogenas), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol.
• Storage: Can be stored at +4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PGD phosphogluconate dehydrogenase [ Homo sapiens ]
• Synonyms: PGD; phosphogluconate dehydrogenase; 6PGD; PGD; 6-phosphogluconate dehydrogenase; EC 1.1.1.44
• Gene ID: 5226
• mRNA Refseq: NM_002631
• Protein Refseq: NP_002622
• MIM: 172200
• UniProt ID: P52209
• Chromosome Location: 1p36.3-p36.13
• Pathway: Glutathione metabolism; Metabolic pathways; Pentose phosphate pathway; Metabolism of carbohydrates
• Function: NADP or NADPH binding; oxidoreductase activity; phosphogluconate dehydrogenase (decarboxylating) activity; protein binding