Recombinant Human Phosphofructokinase, Muscle, His-tagged

• Cat.No.: PFKM-2362H
• Product Overview: Recombinant human PFKM protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. 87.3 kDa (800aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PFKM is regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 5mM DTT 0.2 M NaCl, and 20% glycerol.
• Purity: > 80% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NA AV RAVVRV GIFTGARVFF VHEGYQGLVD GGDHIKEATW ESVSMMLQLG GT VIG SARCK DFREREGRLR AAYNLVKRGI TNLCVIGGDG SLTGADTFRS EWS DLLS DLQ KAGKITDEEA TKSSYLNIVGLVGSIDNDFC GTDMTIGTDS ALHRIMEIVD AIT TT AQSHQ RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE EHL CRRLSET RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ RG GTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSGNQAVRLPLME CV QVTK DVTK AMDEKKFDEA LKLRGRSFMN NWEVYKLLAH VRPPVSKSGS HTVA VMNVGA PAAGMNAAVR STVRIGLIQG NRVLVVHDGF EGLAKGQIEE AGW SYVG GWT GQGGSKLGTK RTLPKKSFEQ ISANITKFNI QGLVIIGGFE AYTGGLELME GRK QFDELCIPFVVIPATVS NNVPGSDFSV GADTALNTIC TTCDRIKQSA AGTK RRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF TIRDLQANVEHLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG IFDSRKNVLG HMQQGGSPTP FDR NF ATKMG AKAMNWMSGK IKESYRNGRIFANTPDSGCV LGMRKRALVF QPVA ELKDQT DFEHRIPKEQ WWLKLRPILK ILAKYEIDLD TSDHAHLEHI TRKRSGEAAV.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PFKM phosphofructokinase, muscle [ Homo sapiens ]
• Synonyms: phosphofructokinase, muscle; GSD7; PFK1; PFKA; PFKX; PFK-1; MGC8699; PFKM; 6-phosphofructokinase, muscle type; PFK-A; phosphohexokinase; phosphofructokinase 1; phosphofructokinase-M; 6-phosphofructo-1-kinase; phosphofructo-1-kinase isozyme A; phosphofructokinase, polypeptide X; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphofructokinase 1; Phosphofructokinase-M; Phosphohexokinase; EC 2.7.1.11
• Gene ID: 5213
• mRNA Refseq: NM_000289
• Protein Refseq: NP_000280
• MIM: 610681
• UniProt ID: P08237
• Chromosome Location: 12q13.3
• Pathway: Fructose and mannose metabolism; Galactose metabolism; Glycolysis / Gluconeogenesis; Metabolic pathways; Pentose phosphate pathway; Diabetes pathways; Integration of energy metabolism; Metabolism of carbohydrates
• Function: 6-phosphofructokinase activity; AMP binding; ATP binding; fructose binding; fructose-6-phosphate binding; identical protein binding; kinase activity; kinase binding; metal ion binding; nucleotide binding; protein C-terminus binding; protein binding; sugar binding; transferase activity