Recombinant Human PHOSPHO1, His-tagged

• Cat.No.: PHOSPHO1-1028H
• Product Overview: Total 295 AA. MW: 31.3 kDa (calculated). N-Terminal His-tag 14 AA (highlighted).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PHOSPHO1 is an ethanolamine derivative that is used to construct sphingomyelins.
• Form: Filtered (0, 4 μm) and lyophilized in 0.5 mg/mL in 0.03 M Acetate buffer, pH 4
• Research topic: Energy metabolism and body weight regulation
• Purity: >95%
• Reconstitution: Add 0.1 M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 μg/mL. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MRGSHHHHHH GMASMSGCFP VSGLRCLSRD GRMAAQGAPR FLLTFDFDET IVDENSDDSI VRAAPGQRLP ESLRATYREG FYNEYMQRVF KYLGEQGVRP RDLSAIYEAI PLSPGMSDLL QFVAKQGACF EVILISDANT FGVESSLRAA GHHSLFRRIL SNPSGPDARG LLALRPFHTH SCARCPANMC KHKVLSDYLR ERAHDGVHFE RLFYVGDGAN DFCPMGLLAG GDVAFPRRGY PMHRLIQEAQ KAEPSSFRAS VVPWETAADV RLHLQQVLKS C
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: PHOSPHO1 phosphatase, orphan 1 [ Homo sapiens ]
• Official Symbol: PHOSPHO1
• Synonyms: PHOSPHO1; phosphatase, orphan 1; phosphoethanolamine/phosphocholine phosphatase; OTTHUMP00000220006; OTTHUMP00000220008; OTTHUMP00000220009
• Gene ID: 162466
• mRNA Refseq: NM_001143804
• Protein Refseq: NP_001137276
• UniProt ID: Q8TCT1
• Chromosome Location: 17q21
• Pathway: Glycerophospholipid metabolism; Metabolic pathways
• Function: hydrolase activity; metal ion binding; phosphoethanolamine/phosphocholine phosphatase activity