Recombinant Human PFKM, His-tagged

• Cat.No.: PFKM-28950TH
• Product Overview: Recombinant full length Human Fructose 6 Phosphate Kinase with a N terminal His tag. 800 amino acids with a predicted MWt 87.3 kDa including tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 780 amino acids
• Description: Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.
• Conjugation: HIS
• Molecular Weight: 87.300kDa inclusive of tags
• Form: Liquid
• Purity: by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 0.08% DTT, 1.17% Sodium chloride, 20% Glycerol
• Storage: Please see Notes section
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF VHEGYQGLVD GGDHIKEATW ESVSMMLQLG GTVIGSARCK DFREREGRLR AAYNLVKRGI TNLCVIGGDG SLTGADTFRS EWSDLLSDLQ KAGKITDEEA TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS ALHRIMEIVD AITTTAQSHQ RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE EHLCRRLSET RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NQAVRLPLME CVQVTKDVTK AMDEKKFDEA LKLRGRSFMN NWEVYKLLAH VRPPVSKSGS HTVAVMNVGA PAAGMNAAVR STVRIGLIQG NRVLVVHDGF EGLAKGQIEE AGWSYVGGWT GQGGSKLGTK RTLPKKSFEQ ISANITKFNI QGLVIIGGFE AYTGGLELME GRKQFDELCI PFVVIPATVS NNVPGSDFSV GADTALNTIC TTCDRIKQSA AGTKRRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF TIRDLQANVE HLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG IFDSRKNVLG HMQQGGSPTP FDRNFATKMG AKAMNWMSGK IKESYRNGRI FANTPDSGCV LGMRKRALVF QPVAELKDQT DFEHRIPKEQ WWLKLRPILK ILAKYEIDLD TSDHAHLEHI TRKRSGEAAV
• Sequence Similarities: Belongs to the phosphofructokinase family. Two domains subfamily.

Gene Information

• Gene Name: PFKM phosphofructokinase, muscle [ Homo sapiens ]
• Official Symbol: PFKM
• Synonyms: PFKM; phosphofructokinase, muscle; PFKX, phosphofructokinase, polypeptide X; 6-phosphofructokinase, muscle type; PFK 1
• Gene ID: 5213
• mRNA Refseq: NM_000289
• Protein Refseq: NP_000280
• MIM: 610681
• Uniprot ID: P08237
• Chromosome Location: 12q13.11
• Pathway: Fructose and mannose metabolism, organism-specific biosystem; Fructose and mannose metabolism, conserved biosystem; Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Glucose metabolism, organism-specific biosystem
• Function: 6-phosphofructokinase activity; 6-phosphofructokinase activity; 6-phosphofructokinase activity; AMP binding; ATP binding