Recombinant Human Peptidylprolyl Lsomerase G (Cyclophilin G)

• Cat.No.: PPIG-1813H
• Product Overview: Recombinant human Cyclophilin G, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW = 21.6 kDa (195aa).

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Peptidyl-prolyl cis-trans isomerase G (PPIG), also known as Cyclophilin G, is a member of peptidyl-prolyl cis-trans isomerase family (PPIases). This protein catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and is implicated in the folding, transport, and assembly of proteins. It is localized to the nuclear speckles, a nuclear compartment rich in splicing factors, and interacts with the splicing factors SC35 and pinin. Cyclophilin G also may play an important role in the regulation of pre-mRNA splicing.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGIKVQRPRC FFDIAINNQP AGRVVFELFS DVCPKTCENF RCLCTGEKGT GKSTQKPLHY KSCLFHRVVK DFMVQGGDFS EGNGRGGESI YGGFFEDESF AVKHNKEFLL SMANRGKDTN GSQFFITTKP TPHLDGHHVV FGQVISGQEV VREIENQKTD AASKPFAEVR ILSCG
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Biological activity: Specific activity is > 200 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.
• Activity Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 1ug of recombinant Cyclophilin G protein to assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25°C.
• Storage: Can be stored at +4°Cshort term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PPIG peptidylprolyl isomerase G (cyclophilin G) [ Homo sapiens ]
• Synonyms: PPIG; peptidylprolyl isomerase G (cyclophilin G); CYP; SRCyp; CARS-Cyp; MGC133241; peptidylprolyl isomerase G; Clk-associating RS-cyclophilin; peptidyl-prolyl isomerase G (cyclophilin G); EC 5.2.1.8; CARS-cyclophilin; CASP10; SR-cyclophilin; SR-cyclophilin; SR-cyp; Cyclophilin G; PPIase G; Peptidyl-prolyl isomerase G; Peptidyl-prolyl cis-trans isomerase G; Rotamase G
• Gene ID: 9360
• mRNA Refseq: NM_004792
• Protein Refseq: NP_004783
• MIM: 606093
• UniProt ID: Q13427
• Chromosome Location: 2q31.1
• Function: cyclosporin A binding; isomerase activity; peptidyl-prolyl cis-trans isomerase activity