Recombinant Human PDGFRB Protein, ENLYFQ-tagged, Alexa Fluor 555 conjugated

• Cat.No.: PDGFRB-471HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human PDGFRB extracellular domain (NP_002600.1) (Met1-Lys531), fused with six amino acids (ENLYFQ) at the C-terminus, was produced in Human Cells.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 506
• Form: Lyophilized
• Molecular Mass: The recombinant human PDGFRβ consists of 506 amino acids and predicts a molecular mass of 57.1 kDa. It migrates as an approximately 92-116 kDa band in SDS-PAGE under reducing conditions.
• N-terminal Sequence Analysis: Leu 33
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: PDGFRB platelet-derived growth factor receptor, beta polypeptide [ Homo sapiens ]
• Official Symbol: PDGFRB
• Gene ID: 5159
• mRNA Refseq: NM_002609
• Protein Refseq: NP_002600
• MIM: 173410
• UniProt ID: P09619