Recombinant Human PDCD1LG2 protein, Avi & His-tagged, Biotinylated

• Cat.No.: PDCD1LG2-1281H
• Product Overview: Biotinylated Human PD-L2 (recommended for biopanning) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Description: Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
• Source: HEK293
• Species: Human
• Tag: His&Avi
• Predicted N Terminal: Leu 20
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 25.2 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Leu 20 - Pro 219
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  No activity loss is observed after storage at:
  4-8°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PDCD1LG2 programmed cell death 1 ligand 2 [ Homo sapiens (human) ]
• Official Symbol: PDCD1LG2
• Synonyms: B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2; B7 dendritic cell molecule; B7-DC; PD-1-ligand 2; PDCD1 ligand 2; butyrophilin B7-DC; programmed death ligand 2; programmed cell death 1 ligand 2
• Gene ID: 80380
• mRNA Refseq: NM_025239.3
• Protein Refseq: NP_079515.2
• UniProt ID: Q9BQ51