Recombinant Human PDCD1 protein, His-tagged

• Cat.No.: PDCD1-839H
• Product Overview: Recombinant Human PDCD1 aa. (Leu41~Ala132) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Leu41~Ala132
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 8.6.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens (human) ]
• Official Symbol: PDCD1
• Synonyms: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; programmed cell death 1 protein; protein PD-1; systemic lupus erythematosus susceptibility 2; programmed cell death protein 1
• Gene ID: 5133
• mRNA Refseq: NM_005018.2
• Protein Refseq: NP_005009.2
• UniProt ID: Q15116