Recombinant Human PCNA, His-tagged

• Cat.No.: PCNA-4863H
• Product Overview: Recombinant full-length human PCNA was expressed by E. coli cells using an N-terminal His tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Full Length
• Description: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.?
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~37 kDa
• Purity: >90%
• Applications: Western Blot
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.2 ug/ul

Gene Information

• Gene Name: PCNA proliferating cell nuclear antigen [ Homo sapiens ]
• Official Symbol: PCNA
• Synonyms: MGC8367; PCNA;?Proliferating cell nuclear antigen; Cyclin;?proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
• Gene ID: 5111
• mRNA Refseq: NM_002592
• Protein Refseq: NP_002583
• MIM: 176740
• UniProt ID: P12004
• Chromosome Location: 20pter-p12
• Pathway: BARD1 signaling events; BRCA1-associated genome surveillance complex (BASC); Base excision repair
• Function: DNA polymerase binding; DNA polymerase processivity factor activity; MutLalpha complex binding