Recombinant Human PARG Protein, His-tagged

• Cat.No.: PARG-1530H
• Product Overview: Recombinant human PARG protein (Met1~Thr976) with a N-terminal His tag was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-976 a.a.
• Description: Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. Several transcript variants encoding different isoforms have been found for this gene.
• Form: Freeze-dried powder
• Molecular Mass: 115 kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Concentration: 200 µg/mL
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PARG poly(ADP-ribose) glycohydrolase [ Homo sapiens (human) ]
• Official Symbol: PARG
• Synonyms: PARG; poly(ADP-ribose) glycohydrolase; PARG99; poly(ADP-ribose) glycohydrolase; mitochondrial poly(ADP-ribose) glycohydrolase; poly(ADP-ribose) glycohydrolase 60 kDa isoform; EC 3.2.1.143
• Gene ID: 8505
• mRNA Refseq: NM_003631
• Protein Refseq: NP_003622
• MIM: 603501
• UniProt ID: Q86W56