Recombinant Human PAK2, GST-His

• Cat.No.: PAK2-721H
• Product Overview: Recombinant Human PAK2, Amino acids D3-R52, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9 cells. MW = 87,954 Da.

Specification

• Cat. No.: PAK2-721H
• Description: The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
• Source: Baculovirus infected Sf9 cells.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: PAK2 was confirmed as PAK2 by specific Western Blotting using anti PAK2 antibody.
• Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
• Concentration: 0.403 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 228 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!
• Protein length: 3-52 a.a.

Gene Information

• Gene Name: PAK2 p21 protein (Cdc42/Rac)-activated kinase 2 [ Homo sapiens ]
• Synonyms: PAK2; p21 protein (Cdc42/Rac)-activated kinase 2; PAK65; PAKgamma; p21-activated kinase 2; S6/H4 kinase; p21 (CDKN1A)-activated kinase 2; EC 2.7.11.1; PAK-2; Gamma-PAK; p58
• Gene ID: 5062
• mRNA Refseq: NM_002577
• Protein Refseq: NP_002568
• MIM: 605022
• UniProt ID: Q13177
• Chromosome Location: 3q29
• Pathway: Axon guidance; ErbB signaling pathway; Focal adhesion; MAPK signaling pathway; Regulation of actin cytoskeleton; Renal cell carcinoma; T cell receptor signaling pathway
• Function: ATP binding; identical protein binding; nucleotide binding; protein serine/threonine kinase activity; transferase activity