Recombinant Human P4HA1, MYC/DDK-tagged

• Cat.No.: P4HA1-3055TH
• Product Overview: Recombinant Human P4HA1 fused with C-terminal MYC/DDK, was expressed in HEK293 Cells.

Specification

• Description: This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
• Source: HEK293 Cells
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 59 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Storage: Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: P4HA1 prolyl 4-hydroxylase, alpha polypeptide I [ Homo sapiens(human) ]
• Official Symbol: P4HA1
• Synonyms: P4HA1; P4HA1; prolyl 4-hydroxylase, alpha polypeptide I; prolyl 4-hydroxylase subunit alpha-1; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I; EC 1.14.11.2
• Gene ID: 5033
• mRNA Refseq: NM_000917
• Protein Refseq: NP_000908
• MIM: 176710
• UniProt ID: P13674
• Chromosome Location: 10q21.3-q23.1
• Pathway: Arginine and proline metabolism
• Function: L-ascorbic acid binding; iron ion binding; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen