Recombinant Human OTUB1 Protein (Met1-Lys271), His tagged

• Cat.No.: OTUB1-4974H
• Product Overview: Recombinant human OTUB1 (Met1-Lys271) fused with His tag was expressed in E. coli.

Specification

• Description: Ubiquitin thioesterase OTUB1, also known as Deubiquitinating enzyme OTUB1, OTU domain-containing ubiquitin aldehyde-binding protein 1, Otubain-1, Ubiquitin-specific-processing protease OTUB1, OTUB1 and OTB1, is a cytoplasm protein which belongs to thepeptidase C65 family. OTUB1 is a hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. OTUB1 is a regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. OTUB1 acts via its interaction with RNF128 / GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform1 of OTUB1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform2of OTUB1 stabilizes RNF128 and promotes anergy. OTUB1 regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1) . OTUB1 mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. OTUB1 is also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 31.62 kDa
• Protein length: Met1-Lys271
• Purity: >90% as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS, pH 7.5 or lyophilized from PBS, pH 7.5.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: OTUB1 OTU domain, ubiquitin aldehyde binding 1 [ Homo sapiens (human) ]
• Official Symbol: OTUB1
• Synonyms: OTUB1; OTU domain, ubiquitin aldehyde binding 1; ubiquitin thioesterase OTUB1; FLJ20113; FLJ40710; otubain-1; OTU-domain Ubal-binding 1; deubiquitinating enzyme OTUB1; ubiquitin-specific protease otubain 1; ubiquitin-specific-processing protease OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTB1; OTU1; HSPC263; MGC4584; MGC111158
• Gene ID: 55611
• mRNA Refseq: NM_017670
• Protein Refseq: NP_060140
• MIM: 608337
• UniProt ID: Q96FW1