Recombinant Human OMA1 Protein, His-tagged

• Cat.No.: OMA1-036H
• Product Overview: Recombinant N-terminal His-tagged OMA1 human protein (217-524) was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 217-524
• Description: Metalloprotease that is part of the quality control system in the inner membrane of mitochondria (PubMed:20038677, PubMed:25605331, PubMed:32132706, PubMed:32132707). Activated in response to various mitochondrial stress, leading to the proteolytic cleavage of target proteins, such as OPA1, UQCC3 and DELE1 (PubMed:20038677, PubMed:25275009, PubMed:32132706, PubMed:32132707). Following stress conditions that induce loss of mitochondrial membrane potential, mediates cleavage of OPA1 at S1 position, leading to OPA1 inactivation and negative regulation of mitochondrial fusion (PubMed:20038677, PubMed:25275009). Also acts as a regulator of apoptosis: upon BAK and BAX aggregation, mediates cleavage of OPA1, leading to the remodeling of mitochondrial cristae and allowing the release of cytochrome c from mitochondrial cristae (PubMed:25275009). In depolarized mitochondria, may also act as a backup protease for PINK1 by mediating PINK1 cleavage and promoting its subsequent degradation by the proteasome (PubMed:30733118). May also cleave UQCC3 in response to mitochondrial depolarization (PubMed:25605331). Also acts as an activator of the integrated stress response (ISR): in response to mitochondrial stress, mediates cleavage of DELE1 to generate the processed form of DELE1 (S-DELE1), which translocates to the cytosol and activates EIF2AK1/HRI to trigger the ISR (PubMed:32132706, PubMed:32132707). Its role in mitochondrial quality control is essential for regulating lipid metabolism as well as to maintain body temperature and energy expenditure under cold-stress conditions (By similarity). Binds cardiolipin, possibly regulating its protein turnover (By similarity). Required for the stability of the respiratory supercomplexes (By similarity).
• Molecular Mass: 37.1 kDa
• Purity: ≥90%
• Applications: WB, ELISA
• Stability: ≥ 1 year
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM Tris, pH 8.0, with 150 mM sodium chloride, 10% glycerol, 0.5 M L-arginine, and 2 μM zinc chloride

Gene Information

• Gene Name: OMA1 OMA1 zinc metallopeptidase [ Homo sapiens (human) ]
• Official Symbol: OMA1
• Synonyms: OMA1; OMA1 zinc metallopeptidase; DAB1; MPRP1; MPRP-1; YKR087C; ZMPOMA1; peptidase; 2010001O09Rik; metalloendopeptidase OMA1, mitochondrial; OMA1 homolog, zinc metallopeptidase; OMA1 zinc metallopeptidase homolog; metalloprotease-related protein 1; overlapping activity with M-AAA protease; overlapping with the m-AAA protease 1 homolog; zinc metallopeptidase OMA1
• Gene ID: 115209
• mRNA Refseq: NM_145243
• Protein Refseq: NP_660286
• MIM: 617081
• UniProt ID: Q96E52