Recombinant Human NTRK3, GST-His

• Cat.No.: NTRK3-702H
• Product Overview: Recombinant Human TRK-C was expressed inSf9insect cells, C-terminal fragment, amino acids V510-G825, activated, N-terminal GST-HIS6 fusion protein with a 3C cleavage site.MW = 64,870 Da.

Specification

• Cat. No.: NTRK3-702H
• Description: TRKC is a member of the TRK family of tyrosine kinase receptors and is the high affinity catalytic receptor for the neurotrophin NT-3 (neurotrophin-3). TRKC mediates the multiple cellular effects of the NT-3 neurotrophic factor, which includes neuronal differentiation and survival. TRKC has been implicated in insulin signaling pathway through interactions with the MUSK protein receptor and the VEGF receptor. Mutations in the TRKC gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers.
• Source: Baculovirus infected Sf9 cells.
• Purification: GST-Affinity Chromatography.
• Product Identity: TRK-C was confirmed as TRK-C by mass spectroscopy LC-ESI-MS/MS.
• Activation: in vitro autoactivation.
• Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
• Specific Activity: 35 pmol/μg×min.
• Concentration: 0.085 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: NTRK3 neurotrophic tyrosine kinase, receptor, type 3 [ Homo sapiens ]
• Synonyms: NTRK3; neurotrophic tyrosine kinase, receptor, type 3; TRKC; gp145 (trkC); ETV6-NTRK3 fusion; OTTHUMP00000192915; neurotrophin 3 receptor; tyrosine kinase receptor C; NT-3 growth factor receptor; ETS related protein-neurotrophic receptor tyrosine kinase fusion protein; EC 2.7.10.1
• Gene ID: 4916
• mRNA Refseq: NM_001007156
• Protein Refseq: NP_001007157
• MIM: 191316
• UniProt ID: Q16288
• Chromosome Location: 15q24-q25
• Pathway: Neurotrophin signaling pathway
• Function: ATP binding; neurotrophin receptor activity; nucleotide binding; protein binding; transferase activity; transmembrane receptor protein tyrosine kinase activity