Recombinant Human NTRK2, GST-His

• Cat.No.: NTRK2-718H
• Product Overview: Recombinant Human TRK-B, C-terminal fragment, amino acids V526-G838, activated, N-terminal GST-HIS6 fusion protein with a Thrombin and 3C cleavage site, expressed inSf9insect cells.MW = 66,818 Da.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: TrkB is the high affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The neurotrophins that activate TrkB are: BDNF (Brain Derived Neurotrophic Factor), NT-4 (neurotrophin-4), and NT-3 (neurotrophin-3). As such, TrkB mediates the multiple effects of these neurotrophic factors, which includes neuronal differentiation and survival.
• Purification: GST-Affinity Chromatography.
• Product Identity: TRK-B was confirmed as TRK-B by mass spectroscopy LC-ESI-MS/MS.
• Activation: in vitro auto-activation.
• Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
• Concentration: 0.133 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 140 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: NTRK2 neurotrophic tyrosine kinase, receptor, type 2 [ Homo sapiens ]
• Synonyms: NTRK2; neurotrophic tyrosine kinase, receptor, type 2; TRKB; GP145-TrkB; OTTHUMP00000021573; OTTHUMP00000021574; OTTHUMP00000021576; OTTHUMP00000021577; tyrosine kinase receptor B; BDNF/NT-3 growth factors receptor; EC 2.7.10.1
• Gene ID: 4915
• mRNA Refseq: NM_001007097
• Protein Refseq: NP_001007098
• MIM: 600456
• UniProt ID: Q16620
• Chromosome Location: 9q22.1
• Pathway: MAPK signaling pathway; Neurotrophin signaling pathway; Signalling by NGF
• Function: ATP binding; neurotrophin receptor activity; nucleotide binding; protein binding; transferase activity; transmembrane receptor protein tyrosine kinase activity