Recombinant human NSDHL, His-tagged

• Cat.No.: NSDHL-2460H
• Product Overview: Recombinant human NSDHL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: NSDHL, as known as Sterol-4-alpha-carboxylate 3-dehydrogenase, is involved in the production (synthesis) of cholesterol. During cholesterol synthesis, it participates in one of several steps that convert a molecule called lanosterol to cholesterol. Specifically, this enzyme removes a carbon atom and three hydrogen atoms (a methyl group) in the conversion of lanosterol to cholesterol. It is also an important component of cell membranes and myelin, the fatty covering that insulates nerve cells.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl
• Molecular Mass: 35.5kDa (320aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEPAVSE PMRDQVARTH LTEDTPKVNA DIEKVNQNQA KRCTVIGGSG FLGQHMVEQL LARGYAVNVFDIQQGFDNPQ VRFFLGDLCS RQDLYPALKG VNTVFHCASP PPSSNNKELF YRVNYIGTKN VIETCKEAGV QKLILTSSAS VIFEGVDIKNGTEDLPYAMK PIDYYTETKI LQERAVLGAN DPEKNFLTTA IRPHGIFGPR DPQLVPILIE AARNGKMKFV IGNGKNLVDF TFVENVVHGHILAAEQLSRD STLGGKAFHI TNDEPIPFWT FLSRILTGLN YEAPKYHIPY
• Purity: >80% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NSDHL NAD(P) dependent steroid dehydrogenase-like [ Homo sapiens ]
• Official Symbol: NSDHL
• Synonyms: NSDHL; NAD(P) dependent steroid dehydrogenase-like; sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating; H105e3; SDR31E1; short chain dehydrogenase/reductase family 31E; member 1; XAP104; protein H105e3; short chain dehydrogenase/reductase family 31E, member 1; H105E3
• Gene ID: 50814
• mRNA Refseq: NM_001129765
• Protein Refseq: NP_001123237
• MIM: 300275
• UniProt ID: Q15738
• Chromosome Location: Xq28
• Pathway: Cholesterol Biosynthesis, organism-specific biosystem; Cholesterol biosynthesis, organism-specific biosystem; Cholesterol biosynthesis, squalene 2,3-epoxide => cholesterol, organism-specific biosystem; Cholesterol biosynthesis, squalene 2,3-epoxide => cholesterol, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: 3-beta-hydroxy-delta5-steroid dehydrogenase activity; nucleotide binding; oxidoreductase activity; sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity