Recombinant Human NRP2 protein(Met1-Tyr855), hFc-tagged

• Cat.No.: NRP2-1047H
• Product Overview: Recombinant Human NRP2 (NP_003863.2)(Met1-Tyr855) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Protein length: Met1-Tyr855
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. 2.Immobilized human VEGFC-His at 10μg/mL (100μL/well) can bind human NRP2-Fc, the EC50 of human NRP2-Fc is 0.1-0.5μg/mL.
• Molecular Mass: The recombinant human NRP2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 1072 amino acids and has a predicted molecular mass of 120.7 kDa. The apparent molecular mass of the protein is approximately 119-129 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NRP2 neuropilin 2 [ Homo sapiens ]
• Official Symbol: NRP2
• Synonyms: NRP2; neuropilin 2; neuropilin-2; VEGF165R2; neuropilin-2b(0); neuropilin-2a(17); neuropilin-2a(22); receptor for VEGF165 and semaphorins class3; vascular endothelial cell growth factor 165 receptor 2; NP2; NPN2; PRO2714; MGC126574
• Gene ID: 8828
• mRNA Refseq: NM_003872
• Protein Refseq: NP_003863
• MIM: 602070
• UniProt ID: O60462