Active Recombinant Human NOS1 protein

• Cat.No.: NOS1-16H
• Product Overview: Recombinant Human NOS1 was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: NOS is responsible for the biosynthesis of nitric oxide from L-arginine in nervous tissues. nNOS is calcium/calmodulin dependent and has a Km = 2.0 μM for L-arginine.
• Form: 40Mm EPS buffer PH7.5 100mMNaCl, 5% glycerol, 5uM H4B, 100uM L-arginine.
• Bio-activity: 350-450 units/mg protein
• Molecular Mass: 160 kDa
• Unit Definition: One unit of enzyme activity represent the amount of enzyme that produce 1nmole of nitric oxide per minute at 37centigrade in 40mM Tris-Hcl, or Hepes or EPPs buffer,PH7.5 containg 5-10 uM Oxyhemoglobin. 1mM NADPH, 2uM FMN/FAD, 5uM H4B, 100uM L-arginine, 50units/ml Catalase, 5units/ml SOD, 0.1mg/ml BSA, 35ul of 9mM EDTA and and 35 ul of 150mg/ml CaM and 12.5mM CaCl2.
• Storage: Store at -70 centigrade

Gene Information

• Gene Name: NOS1 nitric oxide synthase 1 (neuronal) [ Homo sapiens ]
• Official Symbol: NOS1
• Synonyms: NOS1; nitric oxide synthase 1 (neuronal); NOS; nitric oxide synthase, brain; nNOS; NOS type I; neuronal NOS; constitutive NOS; peptidyl-cysteine S-nitrosylase NOS1; bNOS; IHPS1; N-NOS; NC-NOS
• Gene ID: 4842
• mRNA Refseq: NM_000620
• Protein Refseq: NP_000611
• MIM: 163731
• UniProt ID: P29475
• Chromosome Location: 12q14-qter
• Pathway: Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Calcium signaling pathway, organism-specific biosystem
• Function: FMN binding; NADP binding; arginine binding; cadmium ion binding; calmodulin binding; flavin adenine dinucleotide binding; heme binding; metal ion binding; nitric-oxide synthase activity; nitric-oxide synthase activity; oxidoreductase activity; protein binding; tetrahydrobiopterin binding