Recombinant Human NOB1, His-tagged

• Cat.No.: NOB1-2331H
• Product Overview: Recombinant human NOB1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 435aa
• Description: In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Molecular Mass: 49.1 kDa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAPVEHV VADAGAFLRH AALQDIGKNI YTIREVVTEI RDKATRRRLA VLPYELRFKE PLPEYVRLVT EFSKKTGDYP SLSATDIQVL ALTYQLEAEF VGVSHLKQEP QKVKVSSSIQ HPETPLHISG FHLPYKPKPP QETEKGHSAC EPENLEFSSF MFWRNPLPNI DHELQELLID RGEDVPSEEE EEEENGFEDR KDDSDDDGGG WITPSNIKQI QQELEQCDVP EDVRVGCLTT DFAMQNVLLQ MGLHVLAVNG MLIREARSYI LRCHGCFKTT SDMSRVFCSH CGNKTLKKVS VTVSDDGTLH MHFSRNPKVL NPRGLRYSLP TPKGGKYAIN PHLTEDQRFP QLRLSQKARQ KTNVFAPDYI AGVSPFVEND ISSRSATLQV RDSTLGAGRR RLNPNASRKK FVKKR
• Purity: >80% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Store it at +4℃ for short term. For long term storage, store it at -20℃ ~ -70℃ Avoid freeze/thaw cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: NOB1 NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: NOB1
• Synonyms: NOB1; NIN1/RPN12 binding protein 1 homolog (S. cerevisiae); PSMD8 binding protein 1 , PSMD8BP1; RNA-binding protein NOB1; ART 4; MST158; nin one binding protein; NOB1P; protein ART-4; PSMD8 binding protein 1; phosphorylation regulatory protein HP-10; adenocarcinoma antigen recognized by T lymphocytes 4; ART-4; MSTP158; PSMD8BP1
• Gene ID: 28987
• mRNA Refseq: NM_014062
• Protein Refseq: NP_054781
• MIM: 613586
• UniProt ID: Q9ULX3
• Chromosome Location: 16q22.1
• Pathway: Ribosome biogenesis in eukaryotes, organism-specific biosystem; Ribosome biogenesis in eukaryotes, conserved biosystem
• Function: metal ion binding