Recombinant Human NIMA-related kinase 7, His-tagged

• Cat.No.: NEK7-348H
• Product Overview: NEK7, 1-302? aa, Human, His tag, E.coli?

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-302 a.a.
• Description: NEK7 is protein kinase which plays an important role in mitotic cell cycle progression. It is required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. NEK7 is highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Recombinant human NEK7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol
• Molecular Mass: 37 kDa (326aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMDEQSQ GMQGPPVPQF QPQKALRPDM GYNTLANFRI EKKIGRGQFS EVYRAACLLD GVPVALKKVQ IFDLMDAKAR ADCIKEIDLL KQLNHPNVIK YYASFIEDNE LNIVLELADA GDLSRMIKHF KKQKRLIPER TVWKYFVQLC SALEHMHSRR VMHRDIKPAN VFITATGVVK LGDLGLGRFF SSKTTAAHSL VGTPYYMSPE RIHENGYNFK SDIWSLGCLL YEMAALQSPF YGDKMNLYSL CKKIEQCDYP PLPSDHYSEE LRQLVNMCIN PDPEKRPDVT YVYDVAKRMH ACTASS
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NEK7 NIMA (never in mitosis gene a)-related kinase 7 [ Homo sapiens ]
• Official Symbol: NEK7
• Synonyms: NEK7; NIMA (never in mitosis gene a)-related kinase 7; serine/threonine-protein kinase Nek7; nimA-related protein kinase 7; never in mitosis A-related kinase 7
• Gene ID: 140609
• mRNA Refseq: NM_133494
• Protein Refseq: NP_598001
• MIM: 606848
• UniProt ID: Q8TDX7
• Chromosome Location: 1q31.3
• Function: ATP binding; metal ion binding; nucleotide binding; protein serine/threonine kinase activity