Recombinant Human NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae), His-tagged

• Cat.No.: NFU1-665H
• Product Overview: NFU1, 10-254aa Human, His tag, E.coli

Specification

• Description: NFU1 is a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. This protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of bmultiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human NFU1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In Phosphate buffer saline (pH 7.4) containing 20% glycerol 1mM DTT
• Molecular Mass: 29.9 kDa (268aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSGAAAVAA GLRRRFCHML KNPYTIKKQP LHQFVQRPLF PLPAAFYHPV RYMFIQTQDT PNPNSLKFIP GKPVLETRTM DFPTPAAAFR SPLARQLFRI EGVKSVFFGP DFITVTKENE ELDWNLLKPD IYATIMDFFA SGLPLVTEET PSGEAGSEED DEVVAMIKEL LDTRIRPTVQ EDGGDVIYKG FEDGIVQLKL QGSCTSCPSS IITLKNGIQN MLQFYIPEVE GVEQVMDDES DEKEANSP
• Purity: >90 % by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Protein length: 10-254 a.a.

Gene Information

• Gene Name: NFU1 NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: NFU1
• Synonyms: NFU1; NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae); HIRA interacting protein 5 , HIRIP5; NFU1 iron-sulfur cluster scaffold homolog, mitochondrial; CGI 33; NifU; NIFUC; HIRA-interacting protein 5; iron-sulfur cluster scaffold protein; NifU-like C-terminal domain containing; Nfu; HIRIP; MMDS1; CGI-33; HIRIP5; MGC142252; MGC142254
• Gene ID: 27247
• mRNA Refseq: NM_001002755
• Protein Refseq: NP_001002755
• MIM: 608100
• UniProt ID: Q9UMS0
• Chromosome Location: 2p15-p13
• Function: 4 iron, 4 sulfur cluster binding; iron ion binding; protein binding