Recombinant Human Neuroligin1, His-tagged

• Cat.No.: NLGN1-3219H
• Product Overview: Recombinant Human NLGN1 (extracellular domain, Met 1-Val 823), fused with a polyhistidine tag at the C-terminus, was expressed in Human cells.

Specification

• Species: Human
• Source: Human Cells
• Tag: His
• Protein Length: 1-823 a.a.
• Description: Neuroligin-1, also known as NLGN1, is a single-pass type I membrane protein which belongs to the type-B carboxylesterase / lipase family. Neuroligins are cell-adhesion molecules located at the postsynaptic side of the synapse. Neuroligins interact with beta-neurexins and this interaction is involved in the formation of functional synapses. Neurexins and neuroligins are cell adhesion molecules present in excitatory and inhibitory synapses, and they are required for correct neuron network function. These proteins are found at the presynaptic and postsynaptic membranes. Neurexins and neurologins have an essential role in synaptic transmission. Altered neuronal connections during the development of the human nervous system could constitute the basis of the etiology of numerous cases of autism spectrum disorders.
• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 46
• Molecular Mass: The recombinant human NLGN1 consists of 643 amino acids and predictes a molecular mass of 72 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhNLGN1 is approximately 85-95 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles

Gene Information

• Gene Name: NLGN1 neuroligin 1 [ Homo sapiens ]
• Synonyms: neuroligin 1; NLGN1; NL1; KIAA1070; MGC45115; OTTHUMP00000211275; OTTHUMP00000211276; neuroligin-1
• Gene ID: 22871
• mRNA Refseq: NM_014932
• Protein Refseq: NP_055747
• MIM: 600568
• UniProt ID: Q8N2Q7
• Chromosome Location: 3q26
• Pathway: Cell adhesion molecules (CAMs)
• Function: neurexin binding