Recombinant Human NEU1 protein, His-tagged

• Cat.No.: NEU1-580H
• Product Overview: Recombinant Human NEU1 (Q99519) aa. (Ala47~Leu415) fused with His tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter is also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red spot-myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 45kDa; Accurate Molecular Mass: 46kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ala47~Leu415
• Purity: >98%
• Characteristic: Isoelectric point is 5.3.
• Applications: Cell culture; Activity Assays; In vivo assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: NEU1 neuraminidase 1 [ Homo sapiens (human) ]
• Official Symbol: NEU1
• Synonyms: NEU1; neuraminidase 1; NEU; NANH; SIAL1; sialidase-1; G9 sialidase; N-acetyl-alpha-neuraminidase 1; acetylneuraminyl hydrolase; exo-alpha-sialidase;
  lysosomal sialidase; sialidase 1 (lysosomal sialidase)
• Gene ID: 4758
• mRNA Refseq: NM_000434.3
• Protein Refseq: NP_000425.1
• UniProt ID: Q99519