Recombinant Human NECTIN4 protein, hFc-tagged

• Cat.No.: NECTIN4-968H
• Product Overview: Recombinant Human NECTIN4 protein(Q96NY8)(Gly32-Ser349), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Gly32-Ser349
• Tag: C-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Nectin-4, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 61.1 kDa. Due to glycosylation, the protein migrates to 72-74 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.